منابع مشابه
Equilibrium and kinetic studies of the pyruvic kinase reaction.
or indirectly by a multienzyme system consisting of malic enzyme, malic dehydrogenase, and phosphoenolpyruvic carboxykinase (2, 3). However, the physiological significance of these two possible pathways has not been established. Lardy and Ziegler (4) demonstrated that the pyruvic kinase reaction is reversible in the presence of potassium and Meyerhof and Oesper (5) determined the apparent equil...
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The purpose of this investigation was to develop a simple colorimetric method for creatine kinase (CK). The new method is based on the reaction of creatine, formed enzymatically from creatine phosphate and ADP, with different glyoxal compounds. Hydrated glyoxals, such as para-nitrophenyl, 2-thiophene, 4- biphenyl, 4, 4' -biphenyl,α-naphthyl, β-naphthyl, para-chlorophenyl, and styryl were...
متن کاملnovel reagents for creatine kinase
the purpose of this investigation was to develop a simple colorimetric method for creatine kinase (ck). the new method is based on the reaction of creatine, formed enzymatically from creatine phosphate and adp, with different glyoxal compounds. hydrated glyoxals, such as para-nitrophenyl, 2-thiophene, 4- biphenyl, 4, 4' -biphenyl,α-naphthyl, β-naphthyl, para-chlorophenyl, and styryl were s...
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We have investigated the effect of theophylline on the kinetics of the catalytic subunit of protein kinase and related factors in lung tissue. The results show that the point of highest concentration of the C subunit of protein kinase which is active in casein phosphorylation is at 3h of incubation time, but in the presence of 100 Ilg/ InL and 10µg/mL theophylline, this is shifted to I.S an...
متن کاملEffects of univalent cations on the immunoelectrophoretic behavior of pyruvic kinase.
In 1942, Boyer, Lardy, and Phillips' showed that univalent cations were essential for the activity of pyruvic kinase. Since this discovery, the activities of many enzymes catalyzing seemingly unrelated reactions have been shown to depend oil appropriate univalent cation salts. Generally, K+, NH4+, or Rb+ are effective cofactors, Na+ is much less effective if at all, and Li+ and Tris+ fail to ac...
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ژورنال
عنوان ژورنال: Journal of Biological Chemistry
سال: 1960
ISSN: 0021-9258
DOI: 10.1016/s0021-9258(18)69340-8